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Troubleshooting

Here are some of the main causes of DNA sequencing problems and how to identify and overcome them.

Failed Sequencing Reaction

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Multiple Peaks in Data/Mixed Signal

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Primer Dimers

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Short Read Length/Poor Quality Data

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Abrupt Signal Loss

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"Top-Heavy" Data

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Homopolymer Regions

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"Dye-Blobs"

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© Department of Anatomy
University of Otago
PO Box 913
Dunedin 9054
New Zealand

Email gas@anatomy.otago.ac.nz